Mass spectrometry in biodefense
Identifieur interne : 000E22 ( Main/Exploration ); précédent : 000E21; suivant : 000E23Mass spectrometry in biodefense
Auteurs : Plamen A. Demirev [États-Unis] ; Catherine Fenselau [États-Unis]Source :
- Journal of Mass Spectrometry [ 1076-5174 ] ; 2008-11.
English descriptors
- KwdEn :
Abstract
Potential agents for biological attacks include both microorganisms and toxins. In mass spectrometry (MS), rapid identification of potential bioagents is achieved by detecting the masses of unique biomarkers, correlated to each agent. Currently, proteins are the most reliable biomarkers for detection and characterization of both microorganisms and toxins, and MS‐based proteomics is particularly well suited for biodefense applications. Confident identification of an organism can be achieved by top‐down proteomics following identification of individual protein biomarkers from their tandem mass spectra. In bottom‐up proteomics, rapid digestion of intact protein biomarkers is again followed by MS/MS to provide unambiguous bioagent identification and characterization. Bioinformatics obviates the need for culturing and rigorous control of experimental variables to create and use MS fingerprint libraries for various classes of bioweapons. For specific applications, MS methods, instruments and algorithms have also been developed for identification based on biomarkers other than proteins and peptides. Copyright © 2008 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/jms.1474
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000706
- to stream Istex, to step Curation: 000706
- to stream Istex, to step Checkpoint: 000513
- to stream Main, to step Merge: 000E29
- to stream Main, to step Curation: 000E22
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mass spectrometry in biodefense</title><author><name sortKey="Demirev, Plamen A" sort="Demirev, Plamen A" uniqKey="Demirev P" first="Plamen A." last="Demirev">Plamen A. Demirev</name></author><author><name sortKey="Fenselau, Catherine" sort="Fenselau, Catherine" uniqKey="Fenselau C" first="Catherine" last="Fenselau">Catherine Fenselau</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:764A2BBB8F184F2497854994A98596DAA53AC68E</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1002/jms.1474</idno><idno type="url">https://api.istex.fr/document/764A2BBB8F184F2497854994A98596DAA53AC68E/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000706</idno><idno type="wicri:Area/Istex/Curation">000706</idno><idno type="wicri:Area/Istex/Checkpoint">000513</idno><idno type="wicri:doubleKey">1076-5174:2008:Demirev P:mass:spectrometry:in</idno><idno type="wicri:Area/Main/Merge">000E29</idno><idno type="wicri:Area/Main/Curation">000E22</idno><idno type="wicri:Area/Main/Exploration">000E22</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Mass spectrometry in biodefense</title><author><name sortKey="Demirev, Plamen A" sort="Demirev, Plamen A" uniqKey="Demirev P" first="Plamen A." last="Demirev">Plamen A. Demirev</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Applied Physics Laboratory, Johns Hopkins University, Laurel, MD 20723</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Fenselau, Catherine" sort="Fenselau, Catherine" uniqKey="Fenselau C" first="Catherine" last="Fenselau">Catherine Fenselau</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Biochemistry, University of Maryland, College Park, MD</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">College Park (Maryland)</settlement></placeName><orgName type="university">Université du Maryland</orgName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Mass Spectrometry</title><title level="j" type="abbrev">J. Mass Spectrom.</title><idno type="ISSN">1076-5174</idno><idno type="eISSN">1096-9888</idno><imprint><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2008-11">2008-11</date><biblScope unit="volume">43</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="1441">1441</biblScope><biblScope unit="page" to="1457">1457</biblScope></imprint><idno type="ISSN">1076-5174</idno></series><idno type="istex">764A2BBB8F184F2497854994A98596DAA53AC68E</idno><idno type="DOI">10.1002/jms.1474</idno><idno type="ArticleID">JMS1474</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1076-5174</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>bioinformatics</term><term>biological weapons</term><term>biotoxins</term><term>proteomics</term><term>spores</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Potential agents for biological attacks include both microorganisms and toxins. In mass spectrometry (MS), rapid identification of potential bioagents is achieved by detecting the masses of unique biomarkers, correlated to each agent. Currently, proteins are the most reliable biomarkers for detection and characterization of both microorganisms and toxins, and MS‐based proteomics is particularly well suited for biodefense applications. Confident identification of an organism can be achieved by top‐down proteomics following identification of individual protein biomarkers from their tandem mass spectra. In bottom‐up proteomics, rapid digestion of intact protein biomarkers is again followed by MS/MS to provide unambiguous bioagent identification and characterization. Bioinformatics obviates the need for culturing and rigorous control of experimental variables to create and use MS fingerprint libraries for various classes of bioweapons. For specific applications, MS methods, instruments and algorithms have also been developed for identification based on biomarkers other than proteins and peptides. Copyright © 2008 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region><settlement><li>College Park (Maryland)</li></settlement><orgName><li>Université du Maryland</li></orgName></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Demirev, Plamen A" sort="Demirev, Plamen A" uniqKey="Demirev P" first="Plamen A." last="Demirev">Plamen A. Demirev</name></region><name sortKey="Fenselau, Catherine" sort="Fenselau, Catherine" uniqKey="Fenselau C" first="Catherine" last="Fenselau">Catherine Fenselau</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/CyberinfraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E22 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E22 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Ticri/CIDE
|area= CyberinfraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:764A2BBB8F184F2497854994A98596DAA53AC68E
|texte= Mass spectrometry in biodefense
}}
| This area was generated with Dilib version V0.6.25. |  |